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1A). In the ABA-ABAR-WRKY40-ABI5 core signaling complex, WRKY40 acts to suppress ABA signaling, and high levels of ABA promote ABAR-WRKY40 interactions, then activate expression of ABA responsive genes, including the ABA responsive transcription factors ABI5 and ABI4. J. Exp. Bot., 2017. Arabidopsis ABI5 plays a role in regulating ROS homeostasis by activating CATALASE 1 transcription in seed germination Plant Molecular Biology , Apr 2017 Chao Bi , Yu Ma , Zhen Wu , Yong-Tao Yu , Shan Liang , Kai Lu , Xiao-Fang Wang Additionally, NF-YC9 up-regulates expression of the ABI5 gene in response to ABA. These findings show that NF-YC9 may be involved in ABA signaling as a positive regulator and likely functions redundantly together with other NF-YC members, and support the model that the NF-YC9 mediates ABA signaling via targeting to and aiding the ABA-responsive transcription factors such as ABI5. ABI5 affects reactive oxygen species (ROS) homeostasis by affecting CATALASE expression and catalase activity. Furthermore, we showed that ABI5 directly binds to the CAT1 promoter and activates CAT1 expression.

Biochemical interaction assays demonstrated that ABI5 physically interacted with the RIBOSOMAL S6 KINASE2 (S6K2) protein in plant cell. It has been known that ABA INSENSITIVE 5 (ABI5) plays a vital role in regulating seed germination. In the present experiment, we showed that 3-amino-1,2,4-triazole (3-AT) inhibits seed germination of the loss-of-function mutant abi5-1, but promotes seed germination of the ABI5-overexpression transgenic line, and that ABI5 affects reactive oxygen species (ROS) homeostasis. ABI5 overexpression rescued the growth arrest phenotype at low CO 2. Taken together, our results demonstrate that PPC2 and ABI5 are key regulators of plant acclimation to low CO 2, and positively contribute to carbon fixation and metabolism in C 3 plants.

En aba-ökad interaktion mellan pyl6 aba-receptorn med myc2

Furthermore, we showed that ABI5 directly binds to the CAT1 promoter and activates CAT1 expression. Genetic evidence supports the idea that CAT1 functions downstream of ABI5 in ROS signaling during seed germination. ABI5 overexpression rescued the growth arrest phenotype at low CO 2. Taken together, our results demonstrate that PPC2 and ABI5 are key regulators of plant acclimation to low CO 2, and positively contribute to carbon fixation and metabolism in C 3 plants.

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Genetic evidence supports the idea that CAT1 functions downstream of ABI5 in ROS signaling during seed germination. Loss-of-function in ABI5 (abi5) promotes juvenile-to-adult transition, whereas overexpression of ABI5 delays this transition in short days. Genetic analyses indicated that the effect of mir159ab on vegetative phase change is ABI5 dependent. 2020-03-24 Pharmacological assay showed that abi5-1 mutant was insensitive to TOR inhibitor AZD8055, whereas AtABI5 overexpression lines were hypersensitive to AZD8055 in Arabidopsis.

2011), and a ABI5-mediated transactivation was inhibited by overexpression ofabi1-1, the dominant-negative allele of the protein phosphatase ABI1, and by 1-butanol, Participates in ABA-regulated gene expression during seed development and subsequent vegetative stage by acting as the major mediator of ABA repression of 27 Feb 2014 In addition, HY5 binding to the ABI5 promoter is enhanced by ABA treatment, and overexpression of ABI5 restores ABA sensitivity in hy5 22 Apr 2017 (ABI5) in Arabidopsis. The AGL21-overexpressing plants were hypersensitive to ABA, salt, and osmotic stresses during seed germination and ABI5 phosphorylation by MAP kinases positively regulates ABI5 nuclear localization Overexpression of phosphor-null ABI5 in abi5-8 mutant restored the ABA Overexpression of ABI5/ABF binding proteins (AFPs) results in extreme ABA resistance of seeds via multiple mechanisms repressing ABA response, including 2020年9月10日 ABI5 Binding Proteins (AFPs) are a family of negative regulators of bZIP Induced by Transient Overexpression of Arabidopsis thaliana ABI5 ABI5 overexpression has been shown to resemble ABI3 overexpression in that it confers hypersensitivity to ABA inhibition of root growth and promotes slightly 3 Apr 2021 with ABI5 and appear to be involved in mediating stress responses. Induced by Transient Overexpression of Arabidopsis thaliana ABI5 6 Oct 2020 Overexpression of ABI3 and ABI5 simultaneously suppressed the ABA- insensitive phe- notypes of coi1 mutants and JAZ-DJas (JAZ1, JAZ5, and 26 Sep 2000 Transgenic plants overexpressing ABI5 are hypersensitive to ABA, with respect to both germination and vegetative growth. We show that ABA ZFP3 overexpression in the Col-0, abi2-1, and abi4-. 101 backgrounds reduced hypocotyl lengths by.
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50% to 60%, while in abi5-1/ZFP3ox seedlings, hy-. 30 Nov 2019 ABI5 emerges as a critical ABA-signaling component in the regulation of the plant Overexpression of another bZIP TF, ABI5, upregulates FLC. av E Henriksson · 2004 · Citerat av 6 — insensitive loci, ABI3, ABI4 and ABI5, encode transcription factors of the part of the overexpression phenotypes reflects the function of these genes in wild-type. The Arabidopsis DELAY OF GERMINATION 1 gene affects ABSCISIC ACID INSENSITIVE 5 (ABI5) expression and genetically interacts with ABI3 during specific phenotype, but overexpression analysis suggested a role for Oshox4 in gene activity is down-regulated in the abil-1, abi3-1 and abi5-1 mutant lines, Detta bevisar det faktum att komplexet av LtWRKY21, VP1 och ABI5 TaWRKY2 and TaWRKY19 overexpression in Arabidopsis led to more efficient salt, Planttillväxt; Protein Overexpression and Purification; ytterligare information 23, 24, 25, 26 och transkriptionsfaktorer (TFs) ) såsom ABI5 eller AREB1 4 . It has been known that ABA INSENSITIVE 5 (ABI5) plays a vital role in regulating seed germination. In the present study, we showed that inhibition of the catalase activity with 3-amino-1,2,4-triazole (3-AT) inhibits seed germination of Col-0, abi5 mutants and ABI5-overexpression transgenic lines.

2020-03-24 Pharmacological assay showed that abi5-1 mutant was insensitive to TOR inhibitor AZD8055, whereas AtABI5 overexpression lines were hypersensitive to AZD8055 in Arabidopsis.
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En aba-ökad interaktion mellan pyl6 aba-receptorn med myc2

The Arabidopsis DELAY OF GERMINATION 1 gene affects ABSCISIC ACID INSENSITIVE 5 (ABI5) expression and genetically interacts with ABI3 during specific phenotype, but overexpression analysis suggested a role for Oshox4 in gene activity is down-regulated in the abil-1, abi3-1 and abi5-1 mutant lines, Detta bevisar det faktum att komplexet av LtWRKY21, VP1 och ABI5 TaWRKY2 and TaWRKY19 overexpression in Arabidopsis led to more efficient salt, Planttillväxt; Protein Overexpression and Purification; ytterligare information 23, 24, 25, 26 och transkriptionsfaktorer (TFs) ) såsom ABI5 eller AREB1 4 .